快乐之光
找到一些看看适合你吗？要是不适合的话就不用给分了，要是适合的话就……（自己也是找了N久才找到的呢）genetically modified organism (GMO);oligonucleotide microarray;GMO screening;multiplex-PCR;event-specific;integration junction sequence随着转基因农作物检测技术的不断发展，PCR技术已经成为最主要的检测方法之一。但是常规的PCR每次只能扩增一个基因，而单独的多重PCR和常规电泳相结合的技术由于受多重PCR技术自身限制，每次扩增的基因数目也很有限，因此不能满足当前转基因农作物检测的要求。 寡核苷酸芯片是一种在基片上点有若干寡核苷酸探针的基因芯片，每个点都含有序列特异的探针以和感兴趣的基因相互补结合。因此，寡核苷酸芯片可以和多重PCR技术相结合用来同时检测多种目标基因。 在这篇论文中，我们开发了多重PCR技术和DNA芯片技术相结合的方法，在同一个反应中同时检测多个目标基因，以达到检测转基因农作物的目的。 我们设计了两大类芯片，其中一类用来检测样本是否是转基因农作物，另一类用来检测样本来自于哪一种转基因品系。 对于第一类芯片，我们使用了20种探针用以检测转基因农作物，按照具体功能可以分为三种：第一种，根据一些普通元件例如启动子、终止子、报告基因等来检测是否为转基因农作物；第二种，根据目标基因例如抗除草剂或是抗虫害基因来做特定基因的检测确认；第三种，根据物种特异性基因来检测该样本来自于哪种作物。为保证方法的有效性，设立不同的阳性对照和阴性对照来鉴定该实验方法，并且通过常规PCR反应和测序来进一步验证。结果表明这种方法可以快速识别样本是否为转基因农作物，并且花费少，效率提高。这种方法可以检测目前95％以上的转基因作物，并且对于大豆的最低检出率是0.5％，玉米是1％。 对于第二类芯片，根据唯一的、品系特异的宿主与插入片断间连接区域的基因序列，通过多重PCR和寡核苷酸芯片相结合的技术，以达到检测出样本来自于哪种转基因品系的目的。转基因农作物商品大豆(GTS 40-3-2)和六种转基因玉米(MON810，MON863，Bt176，Bt11，GA21和T25)通过这种方法进行检测。结果表明对于这些转基因大豆和玉米，该方法均可以适用。 以上两种芯片被证明是一种新的转基因农作物检测的常规方法。With the increasing development of genetically modified organism (GMO) detection techniques, the Polymerase Chain Reaction (PCR) technique has been the mainstay for GMO detection. And an oligonucleotide microarray is a glass chip to the surface of which an array of oligonucleotides was fixed as spots, each containing numerous copies of a sequence-specific probe that is complementary to a gene of interest. So it is used to detected tens or more targets synchronously. In this research, we developed a multiplex polymerase chain reaction (multiplex-PCR) coupled with a DNA microarray system simultaneously aiming at many targets in a consecutive reaction to detect a GMO. We designed two types DNA microarray, one to detect whether the sample was from GMO, the other to detect which event the sample was from. For the first type, there are a total of 20 probes for detecting a GMO in a DNA microarray which can be classified into three categories according to their purpose: the first for screening GMO from un-transgenic plants based on the common elements such as promoter, reporter and terminator genes; the second for specific gene confirmation based on the target gene sequences such as herbicide-resistance or insect-resistance genes; the third for species-specific genes which the sequences are unique for different plant species. To ensure the reliability of this method, different kinds of positive and negative controls were used in DNA microarray. Commercial GM soybean, maize, rapeseed and cotton were identified by means of this method and further confirmed by PCR analysis and sequencing. The results indicate that this method discriminates between the GMOs very quickly and in a cost-saving and more time efficient way. It can detect more than 95％of currently commercial GMO plants and the limits of detection are 0.5％for soybean and 1％for maize. For the second type, an event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity using multiplex-PCR together with oligonucleotide microarray. Commercial GM soybean (GTS 40-3-2), six GM maize events (MON810, MON863, Bt176, Bt11, GA21, and T25) were detected by this method. The results indicate that it is a suitable method for the identification of these GM soybean and maizes. This method is proved to be a new method for routine analysis of GMOs.这里还有个网站有N多！自己慢慢看吧！只能帮助你到这里了。刘冰制作
Leven小万
With the coming of the 21 century,everyone seems to be "micro-ed"in every corner of the world.Microblog,Wechat,microfilm and micro-contacts...This is a year,in which the microfilm is germinated and flourishing in China,and also the moment that advertising on movie and TV is booming.In recent years,TV advertisings of microfilm style can be seen on both TV and networks.This kind of TV advertising is becoming a hot trend and dominating the ads industry rely on its unique design of plots,ingenious artistic conception,abundant creative concepts and the deep emotion.The essay will analyze the phenomenon from three aspects,and probe into the future of the advertisings of microfilm style.Specifically,the article anaylze the current situation of 21 century advertising media on TV in China,and both reason and background of the rising of the TV advertising of microfilm style.Secondly,it give the advantages of the the latter when comparing the traditional advertising on TV and the one of microfilm style .Finally,it takes some of the mostly photographed and well-reacted TV advertising of microfilm style as the example,and have a precise analysis of the creative concept and the emotional appeal.TV advertising of microfilm style is still on a stage of development and popularizing.And I hope the article may have some reference functions to the research on TV advertising of microfilm style and the thriving and prosperous development of the ads industry in the future.微电影式广告和微电影式电视广告，全部译成了"TV advertising of microfilm style"（不然一会儿这个一会儿那个很怪），而且这是全文唯一不确定的专有名词，如果找到替代的和你认为更好的可以全文替换。排比多的还好，但是句子太长的我也没办法，只能一样长了。
爱吃牛蛙的鱼
毕业论文thesis[英][ˈθi:sɪs][美][ˈθisɪs]n.论文，毕业论文; 论点，论题; 命题; 复数：theses易混淆单词：THESIS例句:1.He completed his doctorate in 1999 with his thesis on the technical subject of structural design. 1999年，朱竞翔获得博士学位，博士论文写的是结构设计的技术问题。2.It is a beguilingly simple thesis, one particularly attractive to the western business executives who have joinedthe china gold rush. 但这是一个具有欺性的简单论点，对参与中国淘金浪潮的西方企业高管尤其有吸引力。3.We have a grand new thesis of the emerging markets. 我们现在得出了一套全新的新兴市场理论。4.The question now is whether the overstretch thesis was wrong or simply premature. 目前问题是，过度扩张说是错误命题还是只是言之过早。5.The thesis is that women still do so badly at work mainly because we are not ambitious enough. 书的主题是：女性的工作表现仍如此糟糕，主要是因为我们不够有雄心。同义词：dissertation[英][ˌdɪsəˈteɪʃn][美][ˌdɪsərˈteɪʃn]n.专题论文，学位论文; 学术演讲; essay[英][ˈeseɪ][美][ˈɛsˌe, ɛˈse]n.散文; 随笔，杂记文; 尝试，企图; 试验; vt.尝试; 试验; 经常说的：English dissertation(英语论文)Graduation thesis(毕业论文)
美味偏执狂
毕业论文的英文翻译是thesis，音标是英 [ˈθi:sɪs] 美 [ˈθisɪs] 。
n.论文，毕业论文;论点，论题;命题
1、There is no empirical evidence to support his thesis.
他的论文缺乏实验证据的支持。
2、How well does this thesis stand up to close examination?
这个命题经得起推敲吗？
3、He has finished his thesis.
他的论文完成了。
4、She's finished writing her thesis.
她那篇论文写出来了。
5、Please write an abstract of this article 〔 thesis 〕.
请写一份这本书〔这篇论文〕的摘要。
The article has a clear-cut thesis and arguments, but lacks reasoning.
文章论点、论据鲜明，但缺乏论证。
绿桑坊蚕被店
As the 21st century unfolds , everyone is "micro" in every corner of the world . Microblogging , micro-channel , micro-film , micro contacts · · · · · · This is a micro- movie flourish in China , began to form the climate of the year , but also the micro-film advertising is generated and the rapid development of the moment. In recent years , we can also on TV on the internet or see micro-film -style television commercials. This type of advertising with its unique plot design , exquisite artistic ideas , as well as a wealth of creative ideas placed deep emotion began in China set off an upsurge , increasingly occupy the leading position in the advertising industry . The articles will be analyzed from three aspects to explore micro- movie-style TV commercials way out. Specifically, the first of the 21 century, the status quo of China's TV advertising media and the rise of micro-film advertising the causes and background ; secondly stresses traditional television advertising and micro- movie -style TV commercials comparative analysis pointed out that the impact of micro-film advertising traditional advertising the advantage ; finally also to appear on several television or the network of high frequency, audience response good micro- movie-style advertising, for example detailed analysis of the creative ideas and emotional demands . Currently micro- movie-style television advertising is still in development, promotion stage. I hope that this type of advertising for the micro-film research can in good health for the future development of the advertising industry has a certain reference.
bluelights
A Thesis Submitted as a Partial Fulfillment of the Requirement for the Degree of B. A./B. S. in ***这是标准的学士学位毕业论文的说法，B.A.代表文学学士，B.S.代表理学学士，***处填上专业。
失忆看星星
转基因技术与传统技术的关系 自从人类耕种作物以来，我们的祖先就从未停止过作物的遗传改良。过去的几千年里农作物改良的方式主要是对自然突变产生的优良基因和重组体的选择和利用，通过随机和自然的方式来积累优良基因。遗传学创立后近百年的动植物育种则是采用人工杂交的方法，进行优良基因的重组和外源基因的导入而实现遗传改良。 因此，转基因技术与传统技术是一脉相承的，其本质都是通过获得优良基因进行遗传改良。但在基因转移的范围和效率上，转基因技术与传统育种技术有两点重要区别。第一，传统技术一般只能在生物种内个体间实现基因转移，而转基因技术所转移的基因则不受生物体间亲缘关系的限制。第二，传统的杂交和选择技术一般是在生物个体水平上进行，操作对象是整个基因组，所转移的是大量的基因，不可能准确地对某个基因进行操作和选择，对后代的表现预见性较差。而转基因技术所操作和转移的一般是经过明确定义的基因，功能清楚，后代表现可准确预期。因此，转基因技术是对传统技术的发展和补充。将两者紧密结合，可相得益彰，大大地提高动植物品种改良的效率。 下面分别对转基因动物和转基因植物来进行描述。Transgenic technology and the relationship between the traditional technology Ever since mankind has been cultivated crops, our ancestors would have never stopped the genetic improvement of crops. Over the past several thousand years the way crop improvement is mainly produced by natural mutation of genes and recombinant excellent selection and use, through the random and natural way to accumulate good genes. Genetics, nearly a hundred years after its founding breeding of plants and animals is the use of artificial hybridization method for the fine and exogenous recombinant genes into genetic improvement achieved. Thus, transgenic technology and conventional technology is the same strain, and its nature through genetic improvement of access to good genes. However, the scope and efficiency of gene transfer, the transgenic technology and conventional breeding techniques There are two important differences. First, the traditional techniques usually only between individuals within a species to achieve gene transfer, while the transgenic technology transfer genes between organisms is not kinship restrictions. Second, the conventional hybridization and selection techniques are generally carried out in the biological individual level, the operation targeted at the whole genome, the diversion of a large number of genes, some genes can not be precisely right to operate and selection, the performance of future generations predictable is poor. The transgenic technology operations and the transfer of a clear definition of the general is the result of gene, function well, and their offspring can accurately forecast the performance of. Thus, transgenic technology is the development of traditional technologies and additions. Closely integrate the two can complement each other greatly enhance the efficiency of plant and animal species improvement. The following were genetically modified animals and transgenic plants to be described.
体验营销在房地产企业中的应用随着体验经济时代的到来，房地产业传统的战略优势，如自然资源、规模经济、资金与技术优势，随着竞争的加剧正在缩小而不再成为必然的优势；企
晴空，朗照 2人参与回答 2023-12-11 不能，知网的引擎是顶级的，只要文章的结构少许类似就会被检测出来。不要在论文这种事上开玩笑。
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HELLO小不不 8人参与回答 2023-12-08 