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1. Vongxay K, Wang SN, Zhang XF, Wu BB, Hu HX, Pan ZJ, Cheng SY, Fang WH. Pathogenetic Characterization of Vibrio parahaemolyticus Isolates from Clinical and Seafood Sources. International Journal of Food Microbiology, 2008, . 王淑娜,方维焕。荧光定量PCR法检测副溶血弧菌tdh基因的表达差异。畜牧与兽医,2008,已录用。3. 陈 阳,吴 迪,陈雪燕,赵焕灿,扈鸿霞,Cheick A Diakite,方维焕。猪链球菌2型分离株对野生斑马鱼的致病性。动物医学进展,2008,已录用。4. 刘刚,李肖梁。犬瘟热病毒H基因的序列分析与表达。畜牧与兽医,2008,已录用。5. 陈健舜, 李肖梁,田国明,马有智,方维焕。检测猪链球菌2型的三重PCR方法建立与应用。中国兽医学报, 2008,已录用。6. Vongxay K, Pan ZJ, Zhang XF, Cheng SY, Mei LL, XU C, Wang SN, Fang WH. Occurrence of pandemic clones of Vibrio parahaemolyticus isolates from seafood and clinical samples in a Chinese coasting province. Foodborne Pathogens and Disease, 2008, 5(2):. Yang ZZ, Shuai JB, Dai XJ, Fang WH. A survey on porcine circovirus type 2 infeciton and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province. Journal of Zhejiang Univ Science B, 2008, 9(2):. Jiang LL, Chen JS, Xu JJ, Zhang XF, Wang SN, Zhao HC, Vongxay K, Fang WH. Virulence characterization and genotypic analyses of Listeria monocytogenes isolates from food and processing environments in eastern China. International Journal of Food Microbiology, 2008, 21(1): . Shuai JB, Li XL, Chen N, Chen CY, Fang WH. Characterization and potential use of truncated PCV2 capsid protein and its polyclonal antibody for diagnosis of PCV2 infections. Acta Microbiologica Sinica, 2008:4891):. Shuai JB, Wei W, Jiang LL, Li XL, Chen N, Zhang ZF, Fang WH. Mapping of nuclear localization signals in open reading frame 2 protein from porcine circovirus type 1. Acta Biochimica et Biophysica Sinica, 2008, 40(1):. Chen N, Hu HX, Zhang ZF, Shuai JB, Fang WH. Genetic diversity of the envelope glycoprotein E2 of classical swine fever virus: Recent isolates branched away from historic and vaccine strains. Veterinary Microbiology, 2007, 127:. Shuai JB, Wei W, Li XL, Chen N, Zhang ZF, Chen XY, Fang WH. Genetic characterization of porcine circovirus type 2 (PCV2) from pigs in high-seroprevalence areas in southeastern China. Virus Genes, 2007, 35:. 陈健舜,江玲丽,方维焕。李斯特菌毒力因子及其进化探讨。微生物学报,2007, 47(4):738-742。14. 徐 程,陈亚波,方维焕。O型口蹄疫病毒结构蛋白VP1的原核表达与抗原性分析。动物医学进展,2007,28(6):14-18。15. 应薇芳,赵焕灿,钱 娅,方维焕。浙江部分地区屠宰场待宰猪链球菌的检测及药敏试验。动物医学进展,2007,28(6):24-26。16. 潘自降,坎 布,沈 飙,娄高明,方维焕。副溶血弧菌海产品和临床分离株的表型及溶血素相关基因分析。微生物学报,2007,47(3):508-511。17. 田国明,李肖梁,陈健舜,陈雪燕,方维焕。单核细胞增多性李斯特菌LMO1847基因在不同应激条件下的表达。畜牧与兽医,39(7):7-10。18. Chen XY, Xu JJ, Shuai JB, Chen JS, Zhang ZF, Fang WH. The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium. International Journal of Food Microbiology,2007,115(3):. Yang ZZ, Habib M, Shuai JB, Fang WH. Detection of PCV2 DNA by SYBR Green I-based quantitative PCR, Journal of Zhejiang University Science B, 2007, 8(3):. Jiang LL, Ke CL, Xu JJ, Chen JS, Chen XY, Chen N, Shuai JB, Fang WH. Listeria monocytogenes mutants carrying Newcastle disease virus F gene fused to its actA and plcB: In vitro expression and immunogenicity in chickens, Acta Biochimica et Biophysica Sinica, 2007, 39(1):57-6621. 陈亚波,徐 程,张桂芝,方维焕。新城疫病毒HN蛋白抗原表位分析及结构域基因原核表达。中国预防兽医学报,2007,29(1):32-3522. Li XL, Shuai JB, Fang WH*. Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk. Journal of Zhejiang University Science B, 2006, 7(11):. Yang ZZ, Fang WH, Habib M. First Results of Detection of PRRSV and CSFV RNA by SYBR Green I-based Quantitative PCR. Journal of Veterinary Medicine B, 2006;53(10):. Li L, Fang WH, Li JR, Huang YW, Yu L. Oral DNA vaccination with the polyprotein gene of infectious bursal disease virus (IBDV) delivered by attenuated Salmonella elicits protective immune responses in chickens. Vaccine, 2006, 24(33-34):. John Dikki M, Chen XY, Chen N, Fang WH*. Attenuated Salmonella typhimurium as a carrier for prokaryotic and eukaryotic expression vectors, Journal of Zhejiang University (Agric. & Life Sci., ed), 2006, 32(3):. Fang WH, Birgitte B, Siegumfeldt H. Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes, FEMS Microbiology Letters, 2006, 258(2): 208-21327. Jiang LL, Xu JJ, Chen N, Shuai JB, Fang WH. Virulence phenotyping and molecular characterization of a low-pathogenicity Listeria monocytogenes isolate from milk, Acta Biochimica et Biophysica Sinica, 2006, 38(4):. 徐晶靓, 江玲丽, 陈 宁, 帅江冰, 方维焕。携带外源基因的重组减毒单核细胞增多性李斯特菌构建与鉴定。微生物学报,2006,46(3):445-450。29. Vongxay KP, Cheng SY, Zhou XY, Shen B, He XL, Zhang GZ, Fang WH Prevalence of Vibrio parahaemolyticus in seafoods and their processing environments as detected by Duplex PCR. Journal of the Science of Food and Agriculture, 2006, 86(12): . 张桂芝,陈亚波,徐 程,张朝政,方维焕。新城疫病毒F蛋白结构域基因原核表达与抗原表位分析。浙江大学学报(农业与生命科学版)2006,32(1):82-87。31. 杨宗照, 方维焕。猪瘟合并猪繁殖呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)感染。中国兽医学报, 2006,26(3):. Zeng HY, Zhang XF, Sun Z, Fang WH. Multiplex PCR identification of Listeria monocytogenes isolates from milk and milk-processing environments. Journal of the Science of Food and Agriculture, 2006, 86(3):. Yang ZG, Sun HX, Fang WH. Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. Vaccine, 2005, 23 (44): . 李肖梁, 尹兆正, 钱娅,朱志刚,方维焕。抗嗜水气单胞菌IgY的提纯及体外抑菌效果研究。浙江大学学报(农业与生命科学版), 2005, 31(4):. 戴贤君, 方维焕。减毒沙门氏菌为载体的口服生长抑素DNA疫苗对草鱼安全性。中国兽医学报, 2005,25(5):. 戴贤君, 方维焕。减毒沙门氏菌为载体的草鱼口服生长抑素DNA疫苗构建及其稳定性。中国兽医学报 , 2005, 25(4):. 马有智,戴贤君,李肖梁,方维焕。表达猪链球菌溶血素基因的减毒沙门氏菌的构建及鉴定。中国兽医学报, 2005,25(5):478-480。38. 陈雪燕, John M. Dikki, 江玲丽, 帅江冰, 方维焕。重组质粒在减毒沙门氏菌中的稳定性及其对细菌侵袭力的影响。微生物学报,2005,45(5):. 贺晓龙, 张桂芝,方维焕。动物性食品中大肠埃希氏菌O157:H7特异性PCR方法的建立。中国兽医科技, 2005, 35(8):. Ke CL, Song HH , Jiang LL, Zeng HY, Zhang GZ, Fang WH. Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein. Journal of Zhejiang University (Agric. & Life Sci., ed), 2005, 31(5): . Jiang LL, Song HH, Chen XY, Ke CL, Xu JJ, Chen N, Fang WH. Characterization of a Mutant Listeria monocytogenes Strain Expressing Green Fluorescent Protein. Acta Biochimica et Biophysica Sinica, 2005, 37(1): 19–2442. Song HH, Wang ZL, Zheng DX, Fang WH, Li Y, Liu YY, Niu ZD, Qiu BS. A novel mucosal vaccine against foot-and-mouth disease virus induces protection in mice and swine. Biotechnology Letters, 2005, 27(21):. Song HH, Fang WH, Wang ZL et al. Detection of foot-and-mouth virus antibodies using a purified protein from the high-level expression of codon-optimized, foot-and-mouth disease virus complex epitopes in Escherichia coli. Biotechnology Letters, 2004, 26(16):1277-128144. 姜中其,陈晓红,方维焕,张应勤,孙建华。规模化猪场仔猪断奶腹泻大肠杆菌耐药性监测。浙江大学学报(农业与生命科学版),2004, 30(5):. 张晓峰,方维焕,程洁,施伟良。李斯特菌选择性增菌培养分离比较研究。中国公共卫生, 2004, 20(6):. 马有智,李肖梁,方维焕。猪链球菌2型溶血素基因PCR快速检测方法研究。浙江大学学报(农业与生命科学版)2004,30(1):78-8247. 李 龙,方维焕, 樊拥军,许 健,方 立,李建荣,于 涟。减毒沙门氏菌为载体在Vero细胞中表达传染性法氏囊病病毒多聚蛋白基因。生物工程学报,2004,20(3):437-440。48. Fang WH, Siegumfeldt H, Budde BB, Jakobsen M. Osmotic stresses led to decreased intracellular pH of Listeria monocytogenes as analyzed by fluorescence ratio-imaging microscopy. Applied & Environmental Microbiology, 2004, 70(5):. Song HH, Zhou L., Fang WH, Li Yong et al. High level expression of codon optimized food-and-mouth disease virus complex epitopes and cholera toxin B subunit chimera in Hansenula polymorpha. Biochemical and Biophysical Research Communications, 2004, 315(1):. Song HH, Li Y., Fang WH, Geng YF et al. Development of a set of expression vectors in Hansenula polymorpha. Biotechnology Letters, 2003, 25:. 谢荣辉,方维焕,李建荣,卢觅佳,于涟。萧山鸡IL-2基因真核表达载体构建及其在VERO细胞中的表达。浙江大学学报,2003,29(6):649-654。52. 梁雪芽,方维焕,江玲丽。鸡新城疫口服DNA疫苗的安全性、稳定性与免疫效力。 生物工程学报,2003,19(1):24-29。53. 马有智,方维焕,柯春林,张晓峰。猪链球菌2型JX分离株的生物学特性。中国兽医学报,2003,23(4):326-328。54. 马有智,方维焕。猪链球菌血清2型JX02株溶血素基因的克隆及序列分析。中国兽医学报, 2003,23(5):460-46255. 方维焕,梁雪芽,李建荣。减毒沙门氏菌为载体传递DNA疫苗诱导对鸡新城疫病毒的免疫保护。中国兽医学报,2003,23(6):527-529。56. 张晓峰,李爱云,方维焕,江玲丽,柯春林。多重聚合酶链反应鉴别单核细胞增生李斯特菌的研究。中华检验医学杂志,2003,26(2)93。57. 张晓峰,方维焕,江玲丽,杜华华。应用聚合酶链反应检测食品中单核细胞增多性李斯特菌。中国预防医学杂志, 2003,37(3):199。58. 方维焕。食品安全标准和监控体系建设需尽快与国际接轨。国际学术动态,2003,1:15-18。59. 张晓峰,程 洁,方维焕。抗李斯特菌免疫机理研究进展。中国人兽共患病杂志,2003,19(5):113-115。60. 方维焕、梁雪芽。减毒沙门氏菌为载体在真核细胞中表达鸡新城疫病毒F蛋白的研究。生物化学与生物物理学报,2002,34(4):488-493。61. Zhong BX, Weng HB, Fang WH. Preparation of protein samples for gel electrophoresis by sequential extraction. Journal of Zhejiang University, Science ed., 2002,3(5). 宋厚辉,方维焕。猪链球菌ZJ株溶血素基因的克隆和序列分析。中国预防兽医学报,2002,24(2):103-10563. 梁雪芽,方维焕。猪生殖与呼吸综合征病毒的分子生物学研究进展。中国兽医科技.2001,31(8).14-1764. 宋厚辉,方维焕。 产气荚膜梭菌主要毒力因子的分子生物学研究进展。预防兽医学进展,2001,3(1):13-16。65. Fang WH, Almeida ., & Oliver . Effects of lactoferrin and milk on adherence of Streptococcus uberis to bovine mammary epithelial cells. American Journal of Veterinary Research, 2000, 3:. Fang WH. & Oliver S. P. Identification of lactoferrin-binding proteins from bovine mastitis causing Streptococcus uberis. FEMS Microbiology Letters, 1999, 176:. Almeida ., Fang WH., & Oliver . Adherence and internalization of Streptococcus uberis to bovine mammary epithelial cells are mediated by host cell proteoglycans. FEMS Microbiology Letters, 1999, 177:. Fang WH., Luther D. A. & Oliver S. P. Protein expression by Streptococcus uberis in co-culture with bovine mammary epithelial cells. FEMS Microbiol. Letters, 1998, 166:. Fang WH., Luther D. A., Almeida R. A. & Oliver S. P. Decreased growth of Streptococcus uberis in milk from mammary glands of cows challenged with the same mastitis pathogen. Journal of Veterinary Medicine B, 1998, 45:. Ali-Vehmas T., Vikerpuur M., Fang WH., and Sandholm M. Giving selenium supplements to dairy cows strengthens the inflammatory response to intramammary infection and induces a growth-suppressing effect on mastitis pathogens in whey. Journal of Veterinary Medicine A, 1997, 44:. Fang WH., Myllys V. & Sandholm M. Resazurin reduction as a function of respiratory burst of bovine polymorphonuclear neutrophils. American Journal of Veterinary Research, 1997, 58(6):. Fang WH. A novel fluorometric method for evaluation of postantibiotic effect on mastitis-causing Staphylococcus aureus and Escherichia coli. Journal of Microbiological Methods, 1996, 26:. Fang WH. Quantification of Staphylococcus aureus and Escherichia coli in the liquid medium by fluorometry and its use in phagocytosis assay. Journal of Appled Bacteriology, 1996, 80:. Koivunen A-L., Maisi P., Fang WH. & Sandholm M. Inhibition of the protease activity in tracheobronchial aspirates of horses with COPD. American Journal of Veterinary Research, 1996, 57(5):. Malbe M., Salonen M., Fang WH., Oopic T., Jalakas M., Klaassen M. & Sandholm M. Disposition of enrofloxacin (BaytrilR) into the udder after intravenous and intra-arterial injections. Journal of Veterinary Medicine A, 1996, 43:. Fang WH., Vikerpuur M. & Sandholm M. Reconstitution of mastitic milk by adding blood plasma and leukocytes into low cell count milk. Veterinary Research (France), 1996, 27:. Fang WH., Shi M., Huang L. & Chen J. Antagonism of the lactic acid bacteria towards Staphylococcus aureus and Escherichia coli on the agar plates and in milk. Veterinary Research (France), 1996, 27:. Fang WH. & Pyorala S. Mastitis-causing Escherichia coli: Serum sensitivity and susceptibility to selected antibacterials in milk. Journal of Dairy Science, 1996, 79:. Fang WH. & Vikerpuur M. Potency of antibacterial drugs in milk as analyzed by the ß-glucuronidase-based fluorometry. Journal of Veterinary Pharmacology & Therapeutics, 1995, 1:. Fang WH., Vikerpuur M. & Sandholm M. A fluorometric ß-glucuronidase assay for analysis of bacterial growth in milk. Veterinary Microbiology, 1995, 46:. Malbe M., Klaassen M., Fang WH., Myllys V., Vikerpuur M., Nyholm K., Sankari S., Suoranta K. & Sandholm M. Comparison of selenite and selenium yeast feed supplements on Se-incorporation, mastitis and leukocyte function in Se-deficient dairy cows. Journal of Veterinary Medicine A, 1995, 42:. Fang WH. & Sandholm M. Inhibition of the proteinase activity in mastitic milk. Journal of Dairy Research, 1995, 64:. Fang WH., Shi M., Chen J., Huang L. & Shao Q. Comparative aspects of bacterial growth in milk and whey. Chinese Veterinary Science (English), 1994, 1(1):. Fang WH., Shi M., Chen J., Huang L. & Shao Q. Turbidometric determination of bacterial growth in whey. Chinese Veterinary Science (English), 1994, 1(1):. Fang WH., Shi M., Huang L. & Shao Q. Growth of lactic acid bacteria, Staphylococcus aureus and Escherichia coli in normal and mastitic milk and whey. Veterinary Microbiology, 1993, 37:. Fang WH., Jiang C. & Liu H. Epidemiological aspects of bovine mastitis and its control in several dairy herds in south-east China. Preventive Veterinary Medicine, 1993, 15(2-3):. Hu SH, Fang WH, Lu HR, et al. Effect Of Teat Dipping And Dry Cow Therapy On Mastitis In A Commercial Dairy-Herd In China. Preventive Veterinary Medicine, 1990, 10:(1-2), 91-96.

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sophialili

音乐治疗作为国内一种相对比较新颖的心理以及生理辅助治疗 方法 ,正越来越多地引起医疗及保健行业的重视和青睐。下文是我为大家搜集整理的关于音乐疗法论文参考的内容,欢迎大家阅读参考!

浅析音乐疗法在ICU中的临床应用

【摘要】目的:探讨音乐疗法做为辅助治疗手段对ICU内的危重症患者疾病治疗,身心康复的作用。方法:选择入住ICU的45例清醒患者,依患者的个人喜好选择音乐,以2次/日,30分钟/次,进行音乐治疗。结果:显效38例,无效5例,因病情突变中断治疗2例。结论:音乐治疗在ICU中的临床应用表明音乐治疗法可调节人的呼吸、循环、内分泌等生理功能,减轻患者的恐惧,焦虑,孤独等心理,融洽医患关系,改善患者的精神状态,缓解疼痛、紧张等情绪,在提高患者舒适度,稳定心率、血压等方面有较好的效果。

【关键词】音乐疗法;ICU;临床应用

音乐治疗学是一门集音乐,医学和心理学为一体的新兴的边缘交叉学科,是音乐在传统艺术欣赏和审美领域之外的应用和发展[1]。四千多年前古埃及就应用音乐为病人减轻疼痛,我国在两千多年前的经典著作《黄帝内经》中也提出五音疗疾。70年代以来音乐疗法已成为医学家们的重要辅助治疗手段,音乐是一种特殊的物质形式,它通过声音的传递引起人们的生理变化[2]。现将音乐疗法在ICU中的临床应用阐述如下:

1临床资料

选择入住ICU中的清醒患者45例,年龄16-78岁,平均年龄56岁,男性23例,女性22例,其中先心病术后10例,妊高症8例,食道癌术后12例,冠心病8例,脑出血术后5例,慢性肾功能异常2例;学历:大学10例,高中12例,初中及以下23例;2例回族,2例蒙古族,余为汉族;信仰____者5例.

2应用方法

应用前准备:播放音乐前需争取患者的同意并向患者说明音乐播放带来的效果,否则会适得其反。

应用中指导:音乐播放开始,嘱病人缓闭双眼,首先从双手开始,吸气时逐渐握紧双拳(约5S)呼气时缓缓放松双拳(约15S)借此可感受紧张与放松的感觉,然后用类似方法从前臂、上臂、脸部、颈部、肩部、背部、胸部、腹部、大腿、小腿、脚等部位肌肉逐渐进行,整个过程随音乐节奏与呼吸密切配合,循环3次。

应用次数时间的选择:每日两次,每次30分钟;第一次应用于中午休息时间,第二次应用于晚上熄灯以后。

音乐内容的选择:个性化选择音乐,根据病人的心理状态及个人对音乐的 爱好 自行选择最佳播放音乐[3]。对于 文化 层次较低,无音乐爱好的老年患者,音乐效果较差,如果根据他们的宗教信仰播放佛经、赞美诗等可取得较好的效果[4]。音乐的选择主要具备如下条件:轻松明快,旋律优美,节奏缓慢。

音量的选择:音乐即要让患者感受到音乐的节奏优美,旋律动人但又不能喧宾夺主,音量的选择尤其重要:音量过大会变成扰人的躁声,使人感到不安,浮躁。声音过小,使人心志得不到满足,声音过轻,使人空虚疲困,兴趣,情致减退,声音过浊,使人心志低下躁动。只有以适宜的心情欣赏适宜的音乐才能达到人乐和谐的境界。音乐的声响控制在患者易接受的范围内,多数研究者认同音量小于70dB,以高出现场声音4-7dB为宜[5]。

3结果

显效38例,无效5例,2例因病情突变中断治疗。无效患者中:先心病术后1例,脑出血术后2例,妊高症1例,食道癌术后1例;高中学历1例,初中及以下4例;回族2例,汉族3例,信仰____者1例,余显效。

4讨论

音乐疗法可稳定患者的血压心率。声波低的柔和音乐会使人感到轻松愉悦。能消除患者的不良情绪。轻松的乐曲能影响大脑右半球,使脑垂体分泌具有止痛作用的内啡肽,降低儿荼酚胺水平,使心率血压下降[6]。

音乐疗法可提供适宜的休养环境。在整个ICU环境中有多种治疗监护仪器的报警声。医护人员的交流声等。这些躁声对意识清醒的患者都是一种恶性刺激,会增加患者的恐惧和紧张心理,音乐不但可以避免躁声对环境的不良刺激,还可以减轻患者的心理创伤[7]。

音乐疗法可促进护患沟通。

病人一踏进医院,面对生活环境的改变,医务人员的谈话,手术风险的担心。自然就产生了恐惧,焦虑,紧张,排斥情绪。这时病人往往不仅渴望更多的医疗信息,还需要一个温馨的医疗环境。通过交谈,了解音乐疗法的科学性、作用、目的,探讨各人音乐喜好,延伸到共同关心和爱好的话题,缩短了患者与护理人员之间的距离,改善了护患关系,同时也真正体现了“以病人为中心”的整体护理内涵。

总之,音乐疗法对病人的心理活动和生理反应有着积极的影响。并且在加强护患沟通,促进病人早日康复,减少医疗纠纷,提高病人满意度方面有着重要的作用。

参考文献

[1]代启霞:音乐对剖宫产妇的心理影响及护理体会,现代医疗卫生,(6):739-740

[2]赵惠敏:潘恒玲,张玉凤,背景音乐在手术中的应用,吉林医学,(10):1266

[3]林羽纶:李丽卿,许乐,音乐疗法在妇产科患者围手术期的应用研究,(12):1298-1299

[4]李淑芬:王祥云,林少虹等音乐疗法对手术患者的影响,神经损伤与功能重建,2006(12)123-24

[5]林启玲:李翠桦:谈手术室对手术患者实施音乐疗法的整体护理。中华临床医学研究杂志,2005,11(7)995-996

[6]吕瑞英:音乐疗法在骨科疼痛病人手术前后护理中的应用,右江医学(6):609

[7]古月芳:陈晓红,刘晓玲:音乐疗法在肝癌介入术中的应用,实用护理杂志(9):131

>>>下页带来更多的音乐疗法论文参考

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