黄芪中黄酮的提取毕业论文

活性明显降低，羟脯氨酸含量显著减少。根据黄芪提取液对光老化无毛小鼠皮肤组织HYPMDA含量及SOD活力的实验可知，黄芪提取液黄酮对sod活性测定的影响有活性明显降低，羟脯氨酸含量显著减少。黄芪提取物(黄芪多糖,黄芪甲甙)纯度：98%分子式：C41H68O14用途：具有增强免疫力，增强能量，圄疲劳，圄突变，保肝，圄制破骨细胞的作用。

目的：提取分离黄芪桂枝五物汤的总黄酮，然后进行药效学实验,为研制出高效低毒、成分基本清楚、作用明确的新型中药复方制剂奠定基础。方法：采用醇提法飞黄芪桂枝五物汤中的总黄酮进行提取，再将提取的总黄酮用血细胞计数法以小鼠的脾细胞细胞培养活细胞数为指标考察其对小鼠的脾细胞的影响，特点在于首次对黄芪桂枝五物汤单个有效成分群之一总黄酮进行了提取，并进行药效学实验，检验其对脾细胞作用，是否能增强小鼠免疫力的功能。并通过不同的给药剂量，探究量效关系。结果：在实验条件下，实验组脾细胞的增殖活性明显高于对照组，各不同浓度梯度中，给药100μg的效果最显著。结论：黄芪桂枝五物汤在本实验条件下可提高小鼠的脾细胞增殖活性，且在一定范围内促进作用有最大值。Objective: total flavonoids extraction and separation of huangqi guizhi wuwu tang, and then the pharmacodynamics experiments, for developed the risk-benefit ratio, composition of basic clear, clear of new Chinese medicine compound preparations. Methods: alcohol formulation fly to extract total flavonoids in huangqi guizhi wuwu tang, and then extracted flavonoids blood cell counting method in mice spleen cells in cell culture in the index of living cells count its influence on mice spleen cells, characterized by huangqi guizhi wuwu tang for the first time in a single one of the effective components group of flavonoids were extracted, and the pharmacodynamics experiments and test its effect on spleen cells, whether can enhance immunity function in mice. And through different drug dosage, explore the concentration-response relationship. Results: under the condition of experiment, the experimental group and proliferation activity of spleen cells was obviously higher than that of control group, the different concentration gradient of dosing mu 100 g of the most significant effect. Conclusion: huangqi guizhi wuwu tang under this experimental condition can increase the spleen cell proliferation activity of mice, and the promoting effect in certain scope has a maximum value.