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菌体湿重达到(WCW),参考文献《基因重组大肠杆菌表达HrpNEcc蛋白的发酵条件及诱导条件优化》我自己做实验的时候,一般收获的湿菌含量在20-30的样子。

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1Riley LW, Remis RS, Helgerson SD, et al. Outbreaks of hemorrhagic colitis associated with a rate Escherichia coli serotype. N Engl J Med, 1983, 308:权太淑,徐建国,范天锐,等.首次从出血性结肠炎病人中分离到O157∶H7大肠杆菌.中华流行病学杂志,1988,9(特刊4号):. Enterohaemorrhagic Escherichic coli infection in Japan. Wkly Epid Rec, 1996,30: PA. Isolation, identification and typing of Vero cytotoxin-producting Escherichia coli O157. PHLS Microbiol Digest, 1994,11: TG, Swerdlow DL, Griffin PM. Escherichia coli O157∶H7 and the hemolytic-uremic syndrome. N Engl J Med, 1995,333: WB, Willshaw GA, Cheasty T, et al. Vero cytotoxin-producing E coli O157 infection associated with farms. Lancet, 1996,347: SM, Salmon RL, Willshaw GA, et al. Haemorrhagic colitis in child after visit to farm visitor centre. Lancet, 1995, 346: U, Thomson-carter FM, Pennington TH. Molecular epidemiology of Escherichia coli O157∶H7 by pulsed-field gel electro-phoresis and comparison with that by bacteriophage typing. J Clin Microbiol, 1996,34: SB, Ratnasm S. Sorbitol-mac conkey medium for detection of Escherichia coli O157∶H7 associated with hemorrhagic colitis. J Clin Microbiol, 1986,23: NV, Doyle MP. Production and characterization of a monoclonal antibody specific for enterohemorrhagic Escherichia coli of serotypes O157∶H7 and O26∶H11. J Clin Microbiol, 1991,29: CH, Vandel NM, Hixon DL. Rapid immunoassay for detection of Escherichia coli O157 directly from stool specimens. J Clin Microbiol, 1996,34: MM, Xu Jianguo, Kaper JB, et al. DNA probe to identify enterohemorrhagic Escherichia coli O157∶H7 and other serotypes that cause hemorrhagic colitis and hemolytic-uremic syndrome. J Infect Dis, 1987,156:徐建国,黄力保,吴纪民.聚合酶链反应检测出血性大肠杆菌.中华医学检验杂志,1995,18: EG, Wells JG, Strockbine NA. Evaluation of commercial latex reagents for identification of O157 and H7 antigens of Escherichia coli. J Clin Microbiol, 1996,34: N, Carter JE, Morrison BJ, et al. Risk factors for the progression of Escherichia coli O157∶H7 enteritis to hemolytic-uremic syndrome. J Pediatr, 1990,116: F, Turgeon JP, Delage G, et al. Randomized, controlled trial of antibiotic therapy for Escherichia coli O157∶H7 enteritis. J Pediatr, 1992,121:299-303.

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